Anti-20S Proteasome Core Subunits Rabbit pAb

Analysis Note

Positive ControlHeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates

Application

Immunoblotting (1:1000-1:10,000, see application references)Immunoprecipitation (see comments)Paraffin Sections (1:100)

General description

This Anti-20S Proteasome Core Subunits Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of 20S Proteasome Core Subunits.

Recognizes the ~25-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7) in human erythrocytes, HeLa cells and 3T3 cells.

Rabbit polyclonal antibody supplied as diluted serum. Recognizes the ~27-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7).

Immunogen

human erythrocyte proteasomes

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Physical form

Serum diluted in PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Irritant (B)